Adhatoda vasica belonging to Acanthaceae family, commonly known as Adosa, Adhatoda vasica Flowers, Antibacterial activity, Antifungal activity, Diffusion. Abstract. The present study aimed to evaluate the in vitro antimicrobial activity of pharmacologically important Adhatoda vasica plant extracts. Antibacterial activity of leaf extract of Adhatoda vasica was studied against Bacillus subtilis, Antibacterial activity was determined by disc diffusion method.

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Extraction Shade dried and coarsely powdered leaves of A. The fraction was further purified and the compound Vasicine was isolated.

Indian Journal of Microbiology. Results and Discussion The ethanol extract of A. International Journal of Cancer. The extract was filtered through Whatman No.

In vitro antibacterial activity of selected medicinal plants from lower Himalayas.

Other constituents include vitamin C, saponins, flavonoids as well as steroids, and fatty acids [ 13 ]. The structure of the isolated compound Vasicine and Vasicine acetate Figure 1.

The emergence of drug-resistant pathogens and the increase in diseases affecting the immune system have greatly intensified the need to investigate new bioactive metabolites for potential pharmaceutical and industrial applications [ 12 ]. Mobile phase A consisted of water with 0.

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Sankar2 and K. Vasicine acetate showed minimum inhibitory concentration values against bacteria: A human adenocarcinoma cancer cell line was obtained from National Institute of Cell Sciences, Pune.

Potent uterine activity of alkaloid vasicine. Ignacimuthu aftivity Shanmugam [ 15 ] have reported the antitubercular activity of Vasicine acetate isolated from A. Support Center Support Center. Table 4 Cytotoxic effect of Vasicine acetate against A lung adenocarcinoma cancer cell line. BHT is used as standard of antioxidant.

Antibacterial activity of adhatoda vasica leaf extract

The solutions were filtered through a membrane filter pore size 0. Preliminary phytochemical and antibacterial screening of crude extract of the leaf of Adhatoda vasica. The authors declare that there is no conflict of interests regarding the publication of this paper.

The estimation of Vasicine acetate content in the extract was performed using linear regression analysis. Two mobile phases A and B were used at flow rate of 1. UV-Visible spectra were taken in methanol on Thermo Fisher instrument. Natural products still remain the most important source for discovery of new and potential drug molecules. Vasicine acetate was obtained by acetylation of Vasicine recovered from A. The required concentrations, The plant is used in the indigenous system of medicine in India and is a well-known expectorant in both Ayurvedic and Unani Systems of Medicine [ 45 ].


Every year, at leastpeople die worldwide from cancer related to their workplace [ 3 ]. Then the residue was crystallised from chloroform to get Vasicine acetate as colorless crystals m.

Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Minimum Inhibitory Concentration MIC Minimum inhibitory concentration studies of the compounds were performed according to the standard reference methods for bacteria [ 17 ] and filamentous fungi [ 18 ].

Evaluation of antioxidant activity of isoferulic acid in vitro. The column was eluted with chloroform and chloroform: The cytotoxicity was determined according to the method of Balachandran et al. Asian Pacific Journal of Tropical Biomedicine.